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Related Experiment Videos

Fluorophotometry with a small animal adapter

S Ishiko1, A Yoshida, N Kitaya

  • 1Department of Ophthalmology, Asahikawa Medical College, Asahikawa, Japan.

Japanese Journal of Ophthalmology
|January 1, 1996
PubMed
Summary

This study validates the Fluorotron Master for ophthalmic research using tree shrews. Optimal parameters were identified for measuring blood-ocular barrier permeability in these promising animal models.

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Area of Science:

  • Ophthalmology
  • Pharmacokinetics
  • Animal Models

Background:

  • Tree shrews are valuable models for ophthalmic research.
  • Accurate measurement of the blood-ocular barrier (BOB) is crucial for understanding ocular diseases.
  • The Fluorotron Master fluorophotometer is a potential tool for BOB permeability assessment.

Purpose of the Study:

  • To evaluate the utility of the Fluorotron Master with a small animal adapter for ophthalmic research.
  • To determine optimal measurement parameters for assessing BOB permeability in tree shrews.
  • To assess the feasibility of using ultrafiltration with minimal blood samples for fluorescein measurement.

Main Methods:

  • Evaluation of the Fluorotron Master with a small animal adapter in tree shrews.

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  • Determination of optimal fluorescein-sodium (fluorescein-Na) concentration, dosage, and measurement time.
  • Assessment of ultrafiltration using hematocrit tubes for protein-unbound fluorescein quantification.

    • Main Results:

    • Optimal fluorescein-Na concentration: 0.5 x 10(-9) to 1.0 x 10(-6) g/mL.
    • Optimal fluorescein-Na dosage: 2 mg/kg.
    • Optimal measurement time: 30 minutes post-injection into ocular compartments.
    • Ultrafiltration with hematocrit tubes allows minimal blood sampling for accurate fluorescein measurement, reducing animal stress.

    Conclusions:

    • The Fluorotron Master with a small animal adapter is a useful tool for ophthalmic research in tree shrews.
    • Established parameters enable reliable estimation of blood-ocular barrier permeability.
    • The method minimizes impact on the animal's condition, supporting its use in disease models.