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Related Experiment Videos

Synaptically evoked prolonged depolarizations in the developing auditory system

V C Kotak1, D H Sanes

  • 1Center for Neural Science, New York University, New York 10003, USA.

Journal of Neurophysiology
|October 1, 1995
PubMed
Summary

Early auditory neurons exhibit prolonged postsynaptic responses to brief glutamatergic stimulation, suggesting a mechanism for developmental plasticity. This occurs even at low activity rates, influencing neuronal development.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Auditory System Research

Background:

  • Synaptic transmission influences neuronal development, but early activity rates are low.
  • Understanding early developmental plasticity mechanisms is crucial for auditory system development.

Purpose of the Study:

  • To investigate a novel, long-lasting postsynaptic response to brief synaptic stimulation in the developing auditory system.
  • To explore the underlying mechanisms and functional implications of this response in early postnatal gerbils.

Main Methods:

  • Whole-cell patch clamp recordings from the lateral superior olive (LSO) in early postnatal gerbils.
  • Stimulation of the excitatory afferent pathway from the cochlear nucleus.
  • Pharmacological manipulation using Ni(+2), tetrodotoxin, CNQX, and a metabotropic glutamate receptor agonist.

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  • In vivo recording of spontaneous auditory neuron activity.

    • Main Results:

    • A prolonged depolarization (PD) was elicited in 60% of LSO neurons by low-frequency synaptic stimulation.
    • PDs lasted 0.5-35 min, reduced input resistance, and were partially reversed by Ni(+2).
    • Metabotropic glutamate receptor activation mimicked PDs, suggesting a role for this pathway and calcium influx.

    Conclusions:

    • Low rates of glutamatergic transmission can elicit a novel form of developmental plasticity in the auditory system.
    • This plasticity may involve metabotropic pathways and prolonged calcium influx, influencing early neuronal development.
    • The findings provide insights into how the developing auditory system adapts to low activity levels.