Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Common pattern of CD44 isoforms is expressed in morphogenetically active epithelia

Q Yu1, B P Toole

  • 1Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|January 1, 1997
PubMed
Summary

This study identifies specific CD44 variants (CD44v3-v10, CD44v4-v10, CD44v6-v10) crucial for embryonic organ development. These CD44 isoforms are prominently expressed in actively developing epithelial tissues, suggesting a key role in morphogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

BMC cancer·2019
Same author

Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody.

Genetics and molecular research : GMR·2014
Same author

Integrative epigenome analysis identifies a Polycomb-targeted differentiation program as a tumor-suppressor event epigenetically inactivated in colorectal cancer.

Cell death & disease·2014
Same author

Optogenetic stimulation of DAergic VTA neurons increases aggression.

Molecular psychiatry·2014
Same author

Description of the novel KIR2DL3*028 allele identified in a southern Chinese Han individual.

Tissue antigens·2014
Same author

Efficacy and safety comparison of add-on therapy with liraglutide, saxagliptin and vildagliptin, all in combination with current conventional oral hypoglycemic agents therapy in poorly controlled Chinese type 2 diabetes.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2014

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • CD44 is a cell surface glycoprotein with numerous isoforms generated by alternative splicing.
  • While CD44s (standard form) binds hyaluronan, ligands for variant isoforms are less understood.
  • CD44 expression is noted in organogenesis, but isoform-specific localization and function remain unclear.

Purpose of the Study:

  • To identify and map the distribution of CD44 isoforms during embryonic mouse organ development.
  • To investigate the role of specific CD44 variants in epithelial-mesenchymal interactions during morphogenesis.

Main Methods:

  • Immunohistochemistry
  • In situ hybridization
  • Reverse transcription-polymerase chain reaction (RT-PCR)

Related Experiment Videos

  • DNA sequencing

    • Main Results:

    • CD44 variants, particularly CD44(v3-v10), CD44(v4-v10), and CD44(v6-v10), are highly expressed in actively developing epithelia at sites of epithelial-mesenchymal interaction (e.g., developing tooth, nose, hair follicle).
    • These variants show a common expression pattern in active epithelia, suggesting a significant role in morphogenesis.
    • CD44s is also prominent in these epithelia, correlating with the absence of hyaluronan, supporting its role in hyaluronan clearance.

    Conclusions:

    • Specific CD44 variants (CD44v3-v10, CD44v4-v10, CD44v6-v10) are key players in embryonic organ development and morphogenesis.
    • The expression patterns indicate distinct roles for CD44 isoforms in developmental processes.
    • CD44s likely functions in regulating hyaluronan levels during organogenesis.