Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Xenopus Pax-6 and retinal development

N Hirsch1, W A Harris

  • 1Department of Biology, University of California, San Diego, La Jolla 92093, USA.

Journal of Neurobiology
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable Home-Use Triglyceride Meter Demonstrates Good Agreement With Plasma Laboratory Measures During Pregnancies Complicated by Metabolic Disease.

Endocrinology, diabetes & metabolism·2026
Same author

Limitations of ice cores in reconstructing temperature seasonality.

Nature·2025
Same author

Cases Treated in the Louisville Marine Hospital.

Western journal of medicine and surgery·2024
Same author

Fractures of the Inferior Maxilla.

The American journal of dental science·2019
Same author

On Filling Carious Cavities between the Back Teeth.

The American journal of dental science·2019
Same author

Nicotine and estrogen synergistically exacerbate cerebral ischemic injury.

Neuroscience·2011
Same journal

The molecular receptive range of an olfactory receptor in vivo (Drosophila melanogaster Or22a).

Journal of neurobiology·2006
Same journal

Consistent dynamics suggests tight regulation of biophysical parameters in a small network of bursting neurons.

Journal of neurobiology·2006
Same journal

Early condition, song learning, and the volume of song brain nuclei in the zebra finch (Taeniopygia guttata).

Journal of neurobiology·2006
Same journal

L1, beta1 integrin, and cadherins mediate axonal regeneration in the embryonic spinal cord.

Journal of neurobiology·2006
Same journal

Singing, but not seizure, induces synaptotagmin IV in zebra finch song circuit nuclei.

Journal of neurobiology·2006
Same journal

Developmental regulation of sensory axon regeneration in the absence of growth cones.

Journal of neurobiology·2006
See all related articles

Pax-6 is crucial for eye development in Xenopus, showing specific expression patterns during retinal development. While necessary, Pax-6 alone is not sufficient for eye formation, as demonstrated by gene misexpression studies.

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • Pax-6 is a key developmental gene conserved across vertebrates.
  • Understanding Pax-6 function is vital for elucidating eye and central nervous system development.

Purpose of the Study:

  • To clone and characterize the Xenopus laevis Pax-6 homolog.
  • To investigate the role of Xenopus Pax-6 in early development and eye formation.
  • To explore the interaction between Pax-6 and the proneural gene NeuroD.

Main Methods:

  • Cloning of Xenopus laevis Pax-6.
  • Analysis of deduced amino acid sequence identity with other vertebrate Pax-6 homologs.
  • Whole-mount in situ hybridization to determine expression patterns during embryonic development.

Related Experiment Videos

  • Misexpression studies of Pax-6 and NeuroD in Xenopus embryos.

    • Main Results:

    • Xenopus Pax-6 shares high sequence identity (95%) with vertebrate homologs.
    • Pax-6 is expressed in early developing eye, forebrain, hindbrain, and spinal cord.
    • Two distinct phases of Pax-6 retinal expression were observed: early pan-retinal and late restricted expression.
    • Misexpression of Pax-6 caused axial defects but not specific eye phenotypes.
    • Ectopic NeuroD expression disrupted Pax-6 expression and eye development.

    Conclusions:

    • Xenopus Pax-6 plays a critical role in eye development.
    • Pax-6 expression is necessary but not sufficient for eye formation in Xenopus.
    • Interactions with other developmental genes, like NeuroD, are essential for proper eye morphogenesis.