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B-cell-derived IL-10: production and function

N Burdin1, F Rousset, J Banchereau

  • 1Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

Methods (San Diego, Calif.)
|January 1, 1997
PubMed
Summary
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B cells secrete Interleukin-10 (IL-10), a cytokine that impacts immunity and inflammation. This review explores B cell IL-10 production, its role in Epstein-Barr Virus (EBV) infection, and its contribution to diseases.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Interleukin-10 (IL-10) is a cytokine that modulates immune responses, inhibiting cell-mediated immunity and inflammation while promoting humoral immunity.
  • B cells are increasingly recognized as a significant source of IL-10, influencing various physiological and pathological processes.

Purpose of the Study:

  • To review studies on B cell-derived IL-10 production and its functional implications.
  • To investigate the role of IL-10 in Epstein-Barr Virus (EBV)-infected B lymphocytes and normal B cell activation.

Main Methods:

  • Analysis of IL-10 production in established B cell lines and primary B lymphocytes.
  • Experimental manipulation using exogenous IL-10, neutralizing antibodies, and co-stimulatory signals (CD40, SAC).
  • Assessment of B cell proliferation, immunoglobulin secretion (IgG, IgA, IgM), and cytokine synergy (IL-6).

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Main Results:

  • Epstein-Barr Virus (EBV) infection induces high levels of human IL-10 production in B lymphocytes, with IL-10 acting as an autocrine growth factor.
  • Normal B cells produce lower levels of IL-10 upon activation; IL-10 synergizes with IL-6 to promote immunoglobulin secretion.
  • Neutralization of IL-10 inhibits immunoglobulin production but not proliferation in CD40-activated B cells.

Conclusions:

  • B cell-derived IL-10 plays a crucial role in EBV-driven B cell growth and immunoglobulin production.
  • IL-10, in synergy with IL-6, is essential for the differentiation and function of activated B cells.
  • Dysregulated B cell IL-10 production may contribute to lymphoproliferative disorders and autoimmune diseases.