Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ascorbic acid determination with an automated enzymatic procedure

W Lee1, S M Roberts, R F Labbe

  • 1Department of Laboratory Medicine, Harborview Medical Center and University of Washington, Seattle 98104-2499, USA.

Clinical Chemistry
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paradoxical Air Embolism Without Patent Foramen Ovale During Craniotomy in the Sitting Position.

Cureus·2019
Same author

Missing canines: a novel aetiology.

Australian dental journal·2018
Same author

Development of the route to the new anti-AIDS drug abacavir: a highlight of academic/industry liaison.

IDrugs : the investigational drugs journal·2005
Same author

beta-peptides as catalysts: poly-beta-leucine as a catalyst for the Juliá-Colonna asymmetric epoxidation of enones.

Chemical communications (Cambridge, England)·2002
Same author

The effect of the primary structure of the polypeptide catalyst on the enantioselectivity of the Juliá-Colonna asymmetric epoxidation of enones.

Chemical communications (Cambridge, England)·2002
Same author

Solid-phase synthesis of oligodeoxynucleotides containing 3'-S-phosphorothiolate linkages.

Nucleosides, nucleotides & nucleic acids·2001
Same journal

From Pooled Cohorts to PREVENT: A Perspective for Clinical Laboratorians.

Clinical chemistry·2026
Same journal

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
See all related articles

Automated plasma ascorbic acid (vitamin C) measurement is now faster and more precise using a new assay on the Roche Fara analyzer. This method offers improved throughput compared to HPLC, with reliable results for antioxidant nutrient analysis.

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Analytical Chemistry

Background:

  • Ascorbic acid (AA), a vital antioxidant nutrient, requires accurate plasma concentration monitoring.
  • Existing methods like HPLC for AA determination can be time-consuming and less precise.
  • There is a need for rapid, automated, and reliable methods for plasma AA analysis.

Purpose of the Study:

  • To develop and validate a new automated procedure for determining plasma ascorbic acid concentration.
  • To utilize a Roche Fara centrifugal analyzer for high-throughput AA measurement.
  • To compare the new method's performance against conventional techniques.

Main Methods:

  • Plasma deproteinization using metaphosphoric acid.
  • Enzymatic oxidation of ascorbic acid to dehydroascorbic acid using AA oxidase.

Related Experiment Videos

  • Chromogenic coupling with o-phenylenediamine and spectrophotometric measurement at 340 nm.
  • Assay validation including linearity, precision, and interference testing.

    • Main Results:

    • The automated procedure demonstrated significantly faster throughput than HPLC methods.
    • Results showed good correlation with conventional methods, indicating high accuracy.
    • The assay exhibited excellent linearity beyond the typical reference range (26.1–84.6 micromol/L).
    • Improved precision was observed compared to existing techniques.
    • Severe hemolysis was identified as the only significant interference.

    Conclusions:

    • The developed automated method provides a rapid, precise, and reliable means for plasma ascorbic acid determination.
    • This new procedure is suitable for routine clinical laboratory use, enhancing antioxidant nutrient monitoring.
    • The method offers advantages in speed and precision over traditional HPLC assays.