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In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
Published on: November 28, 2017
Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis
B L Ortiz1, A M Garcia, A Restrepo
1Molecular Biology Section, Corporación para Investigaciones Biológicas, Medellín, Colombia.
Abstract:
We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis.

