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Updated: Sep 21, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
Published on: June 9, 2017
Pancreatic beta-cell receptors and G proteins coupled to adenylyl cyclase
J C Marie1, G Rosselin, G Skoglund
1Institut National de la Santé et de la Recherche Médicale, INSERM Unité 55, Hôpital Saint-Antoine, Paris, France.
Abstract:
Glucagon and tGLP-1 receptors can be either coexpressed or selectively expressed in beta-cell models. Our results indicate that both these peptides can regulate insulin secretion from beta-cells through their own specific receptors. The finding of a selective expression of G proteins in insulin and glucagon cells indicates a clear difference in their transduction pathways. A key role of the G alpha s family in beta-cell function is further supported by its conserved cell distribution between different species. In conclusion, one could postulate that in the human beta-cells, tGLP-1 and glucagon receptors could mediate their action through different G protein alpha-subunits of the G alpha s family.
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