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[No or little chemotactic activity of staphylococcal substances on macrophages (author's transl)]
Abstract:
Chemotactic effects of staphylococcal substances on macrophages from guinea pigs were studied using modified Boyden-chambers. Filters with an average pore-diameter of 12 micrometer allowed optimal migration of the macrophages. The macrophages were obtained from the peritoneal exsudates 96 h after stimulation with sodium-caseinate (fig. 1) and subsequently concentrated by centrifugation in a ficoll-ronpacon gradient (fig. 2, table 1). Casein had strong chemotactic effects on the macrophages with and without fresh guinea pig serum (fig. 3). Staphylococci, before and after extraction with guanidinium chloride, purified protein A and capsular substances were neither cytotaxic nor cytotaxigenic (in the absence resp. presence of fresh serum). Culture supernatants and staphylococci after incubation with their homologous antiserum proved cytotaxigenic, also lysates of granulocytes from the blood. Lysates of granulocytes from the peritoneal exsudates of guinea pigs were strongly cytotaxigenic for the macrophages.
Insights
Casein strongly attracts macrophages, while staphylococcal components do not. However, activated staphylococci and granulocyte lysates, particularly from peritoneal exudates, exhibit significant cytotaxigenic effects on macrophages.
Area of Science:
- Immunology
- Microbiology
Context:
- Investigating the interaction between staphylococcal substances and host immune cells.
- Utilizing modified Boyden-chambers for studying cell migration.
- Macrophage isolation from guinea pig peritoneal exudates.
Purpose:
- To determine the chemotactic effects of various staphylococcal components on guinea pig macrophages.
- To assess the cytotaxic and cytotaxigenic potential of staphylococcal substances and granulocyte lysates.
Summary:
- Casein demonstrated potent chemotactic activity on macrophages, both with and without serum.
- Purified staphylococcal substances (protein A, capsular) were not cytotoxic or chemotactic.
- Culture supernatants and staphylococci incubated with antiserum, along with granulocyte lysates, were strongly cytotaxigenic for macrophages.
Impact:
- Highlights the specific components and conditions that elicit macrophage chemotaxis and cytotoxicity.
- Provides insights into the immune response mechanisms against Staphylococcus infections.
- Identifies granulocyte lysates as potent stimulators of macrophage activity.