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Related Experiment Videos

Bradykinin increases catecholamine release via B2 receptors

A Dendorfer1, W Häuser, D Falias

  • 1Institute of Pharmacology, Medical University of Lübeck, Germany.

Pflugers Archiv : European Journal of Physiology
|January 1, 1996
PubMed
Summary

Bradykinin stimulates catecholamine release from neural tissues via B2-receptors. This study investigated bradykinin receptor subtypes and their role in noradrenaline and adrenaline secretion using cell lines and rat models.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Endocrinology

Background:

  • Bradykinin is a peptide involved in various physiological processes, including neurotransmission.
  • The precise mechanisms of bradykinin-induced catecholamine release from neural tissues are not fully elucidated.
  • Understanding bradykinin receptor involvement is crucial for exploring potential therapeutic targets.

Purpose of the Study:

  • To investigate the mechanism of bradykinin-induced catecholamine release from neural tissues.
  • To identify the specific bradykinin receptor subtypes (B1 or B2) mediating this effect.
  • To compare the efficacy of different bradykinin receptor antagonists.

Main Methods:

  • Utilized the rat phaeochromocytoma cell line (PC12) to study noradrenaline secretion.

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  • Employed pithed spontaneously hypertensive rats (SHR) for in vivo investigations of catecholamine release.
  • Administered bradykinin and its receptor agonists/antagonists, including HOE 140 and desArg9-[Leu8]-bradykinin, in conjunction with enzyme inhibitors.

    • Main Results:

    • Bradykinin significantly increased noradrenaline release from PC12 cells, primarily through B2-receptor activation.
    • In vivo studies in SHR confirmed bradykinin's ability to enhance catecholamine release, particularly after angiotensin-converting enzyme inhibition.
    • B2-receptor antagonism (HOE 140) blocked bradykinin's effects, while B1-receptor antagonism showed no inhibitory effect and increased adrenaline levels.

    Conclusions:

    • Bradykinin stimulates catecholamine release from PC12 cells, peripheral sympathetic neurons, and chromaffine cells by activating B2-receptors.
    • The adrenal medulla and PC12 cells are sensitive to bradykinin and potentially to non-specific effects of certain kinin antagonists.
    • Findings highlight the critical role of B2-receptors in bradykinin-mediated catecholamine secretion.