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Related Experiment Videos

Isolated liver perfusion using percutaneous methods: an experimental study in the pig

J Harnek1, W Cwikiel, L Bergqvist

  • 1Department of Radiology, University Hospital, Lund, Sweden.

Cardiovascular and Interventional Radiology
|November 1, 1996
PubMed
Summary

Researchers developed a radiological method for isolated liver perfusion in pigs. This technique achieved minimal leakage to the systemic circulation, showing promise for liver research.

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Area of Science:

  • Interventional Radiology
  • Hepatology
  • Animal Models

Background:

  • Isolated liver perfusion is crucial for studying liver function and disease.
  • Developing minimally invasive radiological techniques for liver isolation is an ongoing challenge.

Purpose of the Study:

  • To establish a radiological method for achieving isolated liver perfusion in an experimental animal model.
  • To assess the efficacy and safety of this method by quantifying leakage to the systemic circulation.

Main Methods:

  • Twenty-one pigs underwent liver isolation using transjugular and transfemoral approaches with occlusion balloons in the inferior vena cava, hepatic artery, and portal vein.
  • Portal vein access was achieved via transjugular-transhepatic or percutaneous transhepatic routes.
  • Circulation patency and systemic leakage were evaluated using 99Tcm-labelled albumin and serial blood tests.

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Main Results:

  • Isolated liver circulation was successfully established in all groups.
  • Group 3, utilizing a percutaneous transhepatic portal vein approach, demonstrated significantly less systemic leakage (<10%) compared to Groups 1 and 2.

Conclusions:

  • Radiological methods can successfully achieve isolated liver perfusion in an experimental setting.
  • The developed technique shows potential for minimizing systemic leakage, facilitating further research in liver physiology and therapeutics.