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Related Experiment Videos

Crystal structure of vancomycin

M Schäfer1, T R Schneider, G M Sheldrick

  • 1Institut für Anorganische Chemie, Universität Göttingen, Germany.

Structure (London, England : 1993)
|December 15, 1996
PubMed
Summary
This summary is machine-generated.

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The crystal structure of vancomycin, a crucial antibiotic, was determined using advanced X-ray techniques. This reveals its asymmetric dimer form, essential for binding bacterial cell wall peptides and combating antibiotic resistance.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Crystallography

Background:

  • Vancomycin is a vital glycopeptide antibiotic used as a last resort against resistant bacteria.
  • It functions by binding to D-Ala-D-Ala in nascent bacterial cell walls, compromising cell integrity.
  • Previous attempts to determine vancomycin's crystal structure were hindered by data limitations and complexity.

Purpose of the Study:

  • To determine the high-resolution crystal structure of vancomycin.
  • To elucidate the structural basis for vancomycin's antibiotic activity and dimer formation.
  • To provide insights for future drug design and mechanistic studies.

Main Methods:

  • Low-temperature synchrotron X-ray diffraction data collection.
  • Application of novel ab initio phasing techniques to solve the crystallographic phase problem.

Related Experiment Videos

  • Atomic resolution structure determination of vancomycin.

    • Main Results:

    • The crystal structure of vancomycin was successfully determined at atomic resolution.
    • Vancomycin exists as an asymmetric dimer, facilitating the binding of two D-Ala-D-Ala peptides.
    • Structural details reveal how binding pockets accommodate ligands or are conformationally regulated.

    Conclusions:

    • The determined structure confirms the asymmetric dimer conformation of vancomycin.
    • The dimer structure explains the simultaneous binding of two peptides, crucial for its mechanism.
    • The role of the asparagine sidechain in regulating binding pocket accessibility is proposed.