Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hyper-antigenic variation occurs with human rhinovirus type 17

L J Patterson1, V V Hamparian

  • 1Department of Medical Microbiology and Immunology, Ohio State University and Children's Hospital Research Foundation, Columbus 43205, USA.

Journal of Virology
|February 1, 1997
PubMed
Summary

Researchers explored if human rhinovirus (RV) antigenic variants emerge under immune pressure. Cloning RVs with antisera revealed that human rhinovirus type 17 can generate significant antigenic variants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is it time for a new kind of hospital physician?

BMJ (Clinical research ed.)·2012
Same author

The "STEP-wise" future of adenovirus-based HIV vaccines.

Current medicinal chemistry·2011
Same author

What is the Commission for Health Improvement?

Postgraduate medical journal·2003
Same author

Formation of HIV-1 envelope-hepatitis B core antigen hybrids with high affinity for CD4.

Biochemical and biophysical research communications·2001
Same author

A conformational C4 peptide polymer vaccine coupled with live recombinant vector priming is immunogenic but does not protect against rectal SIV challenge.

AIDS research and human retroviruses·2001
Same author

Cross-protection in NYVAC-HIV-1-immunized/HIV-2-challenged but not in NYVAC-HIV-2-immunized/SHIV-challenged rhesus macaques.

AIDS (London, England)·2000

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Human rhinoviruses (RVs) exhibit diverse serotypes, with 50 of 90 classified into 16 antigenic groups.
  • Antigenic variation in RVs is hypothesized to arise due to immunologic pressure.
  • Understanding RV antigenic drift is crucial for vaccine development and disease control.

Purpose of the Study:

  • To investigate the potential for selecting antigenic variants of human rhinoviruses (RVs) under laboratory conditions mimicking immunologic pressure.
  • To determine if specific RV serotypes are more prone to generating antigenic variants.
  • To characterize the antigenic properties of selected RV variants.

Main Methods:

  • Cloning of 16 plaque-purified RV serotypes in the presence of homologous, polyclonal antisera.

Related Experiment Videos

  • Examination of cloned isolates for antigenic variation using serum cross-neutralization tests with parental and isolate-specific antisera.
  • Characterization of variants as prime strains using specific antisera.

    • Main Results:

    • Only human rhinovirus type 17 (RV-17) yielded significant antigenic variants after cloning under selective pressure.
    • Some RV-17 variants exhibited up to a 500-fold difference in neutralizing antibody titers compared to the parental strain.
    • Further immunoselection of RV-17 variants generated additional variants, some of which no longer met the classification criteria for RV-17.

    Conclusions:

    • Human rhinovirus type 17 is capable of generating major antigenic variants when subjected to immunologic pressure.
    • The study provides experimental evidence supporting the hypothesis that immunologic pressure can drive RV antigenic variation.
    • The emergence of novel antigenic variants highlights challenges in maintaining effective immunity against evolving rhinovirus populations.