Related Experiment Video
Updated: Aug 16, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Comparative sequence analyses of the genes coding for 16S rRNA of Lactobacillus casei-related taxa
K Mori1, K Yamazaki, T Ishiyama
1Systematic Microbiology Laboratory, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Abstract:
The primary structures of the 16S rRNA genes of the type strains of Lactobacillus casei and related taxa were determined by PCR DNA-sequencing methods. The sequences of Lactobacillus casei, Lactobacillus zeae, Lactobacillus paracasei, and Lactobacillus rhamnosus were different. The Knuc values ranged from 0.0040 to 0.0126. On the basis of the Knuc values and the levels of DNA-DNA relatedness among the strains of these species, the L. casei-related taxa should be classified in the following three species: L. zeae, which includes the type strains of L. zeae and L. casei; a species that includes the strains of L. paracasei and L. casei ATCC 334; and L. rhamnosus.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Three-Domain System of Life
Modern Molecular Taxonomy
Applications of Molecular Taxonomy

