Related Experiment Videos
Corynebacterium coyleae sp. nov., isolated from human clinical specimens
G Funke1, C P Ramos, M D Collins
1Department of Medical Microbiology, University of Zürich, Switzerland. funke@immv.unizh.ch
International Journal of Systematic Bacteriology
|January 1, 1997
Summary
A new bacterium, Corynebacterium coyleae, was identified from human clinical samples. This nonlipophilic coryneform bacterium is distinct from other Corynebacterium species based on phenotypic and genotypic analysis.
Area of Science:
- Microbiology
- Bacteriology
- Clinical Microbiology
Background:
- Previously unknown nonlipophilic coryneform bacteria were isolated from human clinical specimens over a five-year period.
- The isolates exhibited unique phenotypic characteristics, including slow acid production from glucose and a positive CAMP reaction.
Purpose of the Study:
- To characterize and classify a novel coryneform bacterium isolated from human clinical specimens.
- To determine if the isolates represent a new species within the genus Corynebacterium.
Main Methods:
- Phenotypic characterization including acid production and CAMP reactions.
- Chemotaxonomic analyses involving cell wall components and fatty acid profiles.
- Genotypic analyses including 16S rRNA gene sequencing and DNA-DNA hybridization.
Main Results:
- The bacterium produced acid from glucose but not maltose or sucrose, and showed a positive CAMP reaction.
- Chemotaxonomic data and G+C content (62-64 mol%) supported assignment to the genus Corynebacterium.
- 16S rRNA gene sequencing and DNA-DNA hybridization confirmed genotypic distinctness from known Corynebacterium species.
Conclusions:
- The phenotypic and genotypic data unambiguously support the establishment of a new species, Corynebacterium coyleae sp.nov.
- Corynebacterium coyleae is proposed as a novel species within the genus Corynebacterium.
- Strain DSM 44184 (= CCUG 35014) is designated as the type strain for Corynebacterium coyleae.