A single vector cloning, mutagenesis and expression system

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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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Published on: June 27, 2017

E M Towler1, J W Stebbins, C Debouck

  • 1Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.

Gene
|December 12, 1996
PubMed

Abstract:

We describe a multipurpose Escherichia coli vector, pOTSf1blue, that can be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authentic protein overexpression and efficient mutagenesis.

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