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Proton currents in human eosinophils

J Schrenzel¹, D P Lew, K H Krause

¹Department of Medicine, University Hospital, Geneva, Switzerland. schrenze@dminov1.hcuge.ch

The American Journal of Physiology

|December 1, 1996

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Human eosinophils possess H+ currents crucial for parasite killing. These currents are activated by intracellular signals and help extrude protons during the respiratory burst, aiding in parasite defense.

Area of Science:

Immunology
Cell Physiology
Electrophysiology

Background:

Eosinophils combat metazoan parasites via a respiratory burst oxidase.
The mechanism of proton (H+) extrusion during this process is not fully understood.

Purpose of the Study:

To investigate the presence and function of H+ conductance in human eosinophils.
To determine if these currents participate in H+ extrusion during the respiratory burst.

Main Methods:

Whole-cell patch-clamp technique applied to human eosinophils.
Manipulation of ion gradients and intracellular conditions to isolate and characterize H+ currents.
Pharmacological inhibition and activation studies.

Main Results:

Related Experiment Videos

Identified a voltage-activated H+ conductance in human eosinophils.
Demonstrated H+ current activation by cytosolic acidification and Ca2+.
Showed Ca2+ effects may involve phospholipase A2 and arachidonic acid.

Conclusions:

Human eosinophils possess significant H+ currents regulated by intracellular signals.
These H+ currents are likely involved in extruding protons generated during the eosinophil respiratory burst.
Findings provide insights into eosinophil-mediated host defense mechanisms.