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Related Experiment Videos

Actin polymerization and depolymerization during apoptosis in HL-60 cells

M G Levee1, M I Dabrowska, J L Lelli

  • 1Section of General Surgery, University of Michigan Medical School, Ann Arbor, USA.

The American Journal of Physiology
|December 11, 1996
PubMed
Summary

Apoptosis involves cytoskeletal changes, specifically early filamentous (F)-actin polymerization followed by depolymerization, which is crucial for apoptotic body formation. This actin reorganization is essential for the morphological features of programmed cell death.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • The biochemical mechanisms driving apoptosis morphology remain largely unknown.
  • The cytoskeleton, particularly actin, is implicated in apoptosis but its precise role is unclear.

Purpose of the Study:

  • To investigate the role of filamentous (F)-actin and microfilament organization in apoptosis.
  • To elucidate the biochemical machinery underlying apoptotic morphological changes.

Main Methods:

  • Flow cytometry
  • Polyacrylamide gel electrophoresis
  • Fluorescence microscopy
  • Dihydrocytochalasin B (H2CB) treatment

Main Results:

  • Apoptosis induced by UV or etoposide correlated with transient F-actin polymerization and subsequent depolymerization.

Related Experiment Videos

  • F-actin depolymerization preceded apoptotic body formation and affected both soluble and insoluble pools.
  • H2CB blocked apoptotic body formation by depolymerizing F-actin in the detergent-insoluble pool, altering microfilament structure.

    • Conclusions:

    • Microfilament network reorganization is necessary for apoptotic body formation.
    • F-actin depolymerization is a key component in the process of apoptosis.
    • Cytoskeletal dynamics play a critical role in the morphological execution of programmed cell death.