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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid
P G McIntyre1, J Laszlo, K Appleyard
1Department of Medical, Microbiology, Ninewells Hospital, Dundee, UK.
Abstract:
Samples of oral fluid collected from 18 patients seropositive for hepatitis C virus (HCV) and 49 seronegative patients were tested for the presence of HCV antibodies with two modified serum-screening kits. The following sensitivities and specificities were obtained: HCV 3.0 assay, 72% and 98%, respectively, and Monolisa anti-HCV assay, 100%, and 100%, respectively. The modified Monolisa assay demonstrated a striking concordance between serum and saliva samples. The use of oral fluids offers a convenient and noninvasive method applicable to HCV epidemiological studies and screening of high-risk groups.
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Enzyme-Linked Immunosorbent Assay
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Hepatitis