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Cooperative MetR binding in the Escherichia coli glyA control region
1Department of Microbiology, University of Iowa, Iowa City 52242, USA.
FEMS Microbiology Letters
|April 1, 1996
Abstract:
We determined the relative binding affinity of the MetR protein for wild-type and mutant MetR binding sites 1 and 2 in the Escherichia coli glyA control region. The results show that MetR binding site 1 has a higher affinity for the MetR protein than binding site 2. In addition, the results suggest that binding of MetR to the glyA promoter is cooperative. Mutations that decrease the ability of MetR to bind to either site 1 or site 2 have no significant effect on MetR's ability to bend DNA.