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Construction and rapid screening of a representative yeast artificial chromosome library from the Plasmodium
A A Camargo1, K Fischer, M Lanzer
1Zentrum für Infektionsforschung der Universität Würzburg, Germany.
Parasitology Research
|January 1, 1997
Abstract:
Large genomic DNA fragments from the Plasmodium falciparum clone Dd2 have been cloned as artificial chromosomes in yeast (YAC). The resulting library has a 10-fold redundancy for single-copy genes and consists of 1440 individual clones, including 240 telomeric clones, with an average insert size of 150 kb. A novel hybridization method was developed for the rapid and cost-effective screening of protozoan YAC libraries. The Dd2 YAC clones will facilitate a positional approach to the parasite's genes and aid in the dissection of genetic loci associated with the virulence and pathogenicity of P. falciparum.