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Related Experiment Videos

Mouse U2af1-rs1 is a neomorphic imprinted gene

A Nabetani1, I Hatada, H Morisaki

  • 1Department of Bioscience, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.

Molecular and Cellular Biology
|February 1, 1997
PubMed
Summary

The mouse U2af1-rs1 gene, located within the Murr1 gene intron, shows unique genomic imprinting. This imprinted gene is exclusively transcribed from the paternal allele, suggesting a distinct origin and imprinting mechanism.

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Area of Science:

  • Genetics
  • Genomic Imprinting
  • Epigenetics

Background:

  • The U2af1-rs1 gene is an endogenous imprinted gene found on mouse chromosome 11.
  • It is transcribed solely from the unmethylated paternal allele, while the maternal allele remains silent due to methylation.

Purpose of the Study:

  • To investigate the genomic structure and imprinting characteristics of the mouse U2af1-rs1 gene.
  • To understand the relationship between U2af1-rs1 and its surrounding genomic region, particularly the Murr1 gene.

Main Methods:

  • Analysis of mouse genome structure.
  • Allele-specific methylation analysis.
  • Gene transcription analysis.

Main Results:

  • The U2af1-rs1 gene is fully contained within an intron of the Murr1 gene.

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  • The Murr1 gene is biallelically transcribed and not imprinted in neonatal mice.
  • Allele-specific methylation is confined to the region surrounding U2af1-rs1 within the Murr1 intron.

    • Conclusions:

    • The U2af1-rs1 gene exhibits distinct genomic imprinting characteristics compared to its surrounding genomic environment.
    • Results support a proposed neomorphic origin of U2af1-rs1 via retrotransposition.
    • A unique imprinting mechanism for ectopic genes is suggested.