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Related Experiment Videos

Neutrophil rheologic changes in septic shock

P C Yodice1, M E Astiz, B M Kurian

  • 1Department of Medicine, Saint Vincent's Hospital and Medical Center, New York, New York 10011, USA.

American Journal of Respiratory and Critical Care Medicine
|January 1, 1997
PubMed
Summary

Neutrophil function is impaired in severe sepsis and septic shock, with reduced deformability and increased aggregation contributing to microvascular occlusion and decreased blood flow.

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Area of Science:

  • Hematology
  • Critical Care Medicine
  • Immunology

Background:

  • Septic shock is associated with reduced capillary blood flow, potentially due to altered neutrophil rheology and microvascular occlusion.
  • Understanding neutrophil behavior is crucial for addressing impaired blood flow in sepsis.

Purpose of the Study:

  • To investigate neutrophil deformability, adherence, and aggregation in patients with severe sepsis and septic shock.
  • To explore the role of these neutrophil properties in the pathophysiology of septic shock.

Main Methods:

  • Isolated neutrophils from patients with septic shock (SS), severe sepsis (S), critically ill non-infected patients (CINS), and normal volunteers (N).
  • Assessed neutrophil deformability via filtration, aggregation using aggregometry/leukergy, and adherence by latex bead binding.

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  • Utilized cytochalasin D and anti-CD11/CD18 for functional analysis of neutrophils.

    • Main Results:

    • Patients with severe sepsis and septic shock exhibited significantly decreased neutrophil filterability compared to controls.
    • Neutrophil aggregation was notably increased in septic shock patients.
    • Anti-CD11/CD18 treatment improved neutrophil filtration and reduced aggregation in septic shock neutrophils.
    • Neutrophil adherence was not significantly altered in sepsis or septic shock patients.

    Conclusions:

    • Neutrophil deformability is compromised in severe sepsis and septic shock.
    • Increased neutrophil aggregation and potentially leukoaggregation contribute to reduced neutrophil filterability in septic shock.
    • These rheological changes in neutrophils may play a significant role in the impaired microvascular blood flow observed in septic shock.