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Related Experiment Videos

Threshold level for theophylline in doping analysis

F T Delbeke1, P De Backer

  • 1Vakgroep Farmacologie, Farmacie en Toxicologie, Faculteit Diergeneeskunde, Universiteit Gent, Salisburylaan, Merelbeke, Belgium.

Journal of Chromatography. B, Biomedical Applications
|December 6, 1996
PubMed
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This study developed an HPLC method to detect theophylline in athlete urine samples. Proposed decision limits aim to prevent false-positive doping control results, enhancing anti-doping accuracy.

Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Sports Science

Background:

  • Methylxanthines, including theophylline, are common in various substances.
  • Accurate detection of theophylline in athlete urine is crucial for doping control.
  • Existing detection methods require refinement for reliable anti-doping analysis.

Purpose of the Study:

  • To establish a reliable HPLC method for quantifying theophylline and related methylxanthines in urine.
  • To determine appropriate decision limits for theophylline and its metabolite ratios in doping control.
  • To differentiate between dietary and non-dietary theophylline intake in athletes.

Main Methods:

  • Reversed-phase High-Performance Liquid Chromatography (HPLC) was employed for methylxanthine analysis.

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  • Calibration curves demonstrated linearity within the 0-10 µg/ml range.
  • Urine samples from athletes undergoing unannounced doping control were analyzed.

    • Main Results:

    • The limit for accurate theophylline quantitation was determined to be 0.25 µg/ml.
    • Theophylline was detected above 0.25 µg/ml in 1539 out of 3885 athlete urine samples.
    • A theophylline to paraxanthine ratio (TP/PX) was proposed as a more reliable indicator of non-dietary intake.

    Conclusions:

    • The developed HPLC method provides accurate quantification of theophylline in urine.
    • Proposed decision limits of 5 µg/ml for theophylline and 0.50 for the TP/PX ratio can improve the reliability of doping controls.
    • These measures aim to minimize false-positive results in anti-doping efforts.