Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved method for counting virus and virus like particles

Y Z Zheng1, R Webb, P F Greenfield

  • 1Department of Chemical Engineering, University of Queensland, Brisbane, Australia.

Journal of Virological Methods
|December 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Symptom presentation of childbirth-related post-traumatic stress in 31 countries: the INTERSECT study.

Journal of affective disorders·2026
Same author

Erratum: "Interaction potentials of the RG-I anions, neutrals, and cations (RG = He, Ne, Ar)" [J. Chem. Phys. 122, 194311 (2005)].

The Journal of chemical physics·2024
Same author

Development and characterisation of mgTHP-1, a novel <i>in vitro</i> model for neural macrophages with microglial characteristics.

Neurological research·2023
Same author

On the relevance of cocaine detection in a fingerprint.

Scientific reports·2020
Same author

Use of a suction tube to prevent nasal pressure sores during nasotracheal intubation.

The British journal of oral & maxillofacial surgery·2019
Same author

Demonstration of Single-Barium-Ion Sensitivity for Neutrinoless Double-Beta Decay Using Single-Molecule Fluorescence Imaging.

Physical review letters·2018

A new electron microscopy (EM) method accurately counts virus particles. This technique simplifies particle quantification from cell cultures, offering a rapid and reproducible approach for virology research.

Area of Science:

  • Virology
  • Microscopy
  • Biotechnology

Background:

  • Accurate quantification of virus particles is crucial for understanding viral replication and disease pathogenesis.
  • Conventional electron microscopy (EM) methods for particle counting can be labor-intensive and may lack precision.

Purpose of the Study:

  • To develop and validate an improved, simplified method for counting virus and virus-like particles (VLPs) using electron microscopy.
  • To establish a rapid, accurate, and reproducible technique for absolute particle concentration determination.

Main Methods:

  • Developed a method involving even deposition of particles onto EM grids via centrifugation or antibody capture.
  • Utilized a Microfiche unit for particle counting from low-magnification EM images (2500x, 50x enlargement).

Related Experiment Videos

  • Validated the technique using latex particles of known concentration and recombinant Bluetongue virus (BTV) core-like particles (CLPs).

    • Main Results:

    • The Microfiche unit method demonstrated good agreement when compared to conventional counting methods.
    • The technique proved accurate and reproducible for counting both pure particles and those from crude cell culture extracts.
    • Successful application demonstrated with both centrifugation and antibody-based sample loading.

    Conclusions:

    • The developed Microfiche unit-based EM method offers a simple, accurate, and rapid approach for virus particle quantification.
    • This technique enhances reproducibility and is applicable to various sample types, including crude extracts.
    • The method provides a valuable tool for virological studies requiring precise particle enumeration.