Enzymatic substrate recycling electrodes

A weak chemical signal might result in a large response when biochemically amplified. Enzymatic recycling of the analyte is one of the biochemical ways of providing an effective increase in biosensor sensitivity by several orders of magnitude. The enhancement of sensitivity is provided by consecutive consumption and generation of the analyte on the sensor surface. The principle of enzymatic substrate regeneration using bioelectrocatalysis and coupled enzymes is shortly reviewed and illustrated with some recent developments of biosensors for catecholamines, and its potential for electrochemical immunoassays is outlined.