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G2-and early-M-specific expression of the NTCYC1 cyclin gene in Nicotiana tabacum cells

L X Qin1, C Perennes, L Richard

  • 1Institut de Biotechnologie des Plantes, Université Paris-Sud, Plateau du Moulon, Orsay, France.

Plant Molecular Biology
|December 1, 1996
PubMed

Insights

Tobacco mitotic cyclin NTCYC1 is expressed during the G2/M phases of the cell cycle. Its expression correlates with G2 arrest in plant cells, suggesting a role in cell cycle regulation.

Area of Science:

  • Plant molecular biology
  • Cell cycle regulation

Background:

  • Previously isolated NTCYC1, a tobacco mitotic cyclin cDNA.
  • Investigated expression patterns of NTCYC1 and Ntsuc1 (a suc 1 plant homologue) in synchronized tobacco cells.

Purpose of the Study:

  • Describe expression patterns of NTCYC1 and Ntsuc1 in synchronized tobacco cells.
  • Compare NTCYC1 expression with Ntcdc2-1 (a cdc2 homologue).
  • Investigate the role of NTCYC1 in plant cell cycle control and G2/M transition.

Main Methods:

  • Synchronization of tobacco cell suspensions.
  • Analysis of NTCYC1, Ntsuc1, and Ntcdc2-1 expression patterns via transcript detection.
  • Microinjection of NTCYC1 mRNA into Xenopus oocytes for functional analysis.
  • Assessment of N. tabacum mesophyll cell status (DNA content) and NTCYC1 transcript levels in relation to leaf position.

Main Results:

  • NTCYC1 transcript detected only in late G2 and early M phases; absent in G1 and S phases.
  • Ntsuc1 and Ntcdc2-1 showed constitutive expression throughout the cell cycle.
  • Microinjection experiments suggested NTCYC1's role in controlling the G2/M transition.
  • A population of mesophyll cells with 4C DNA content (G2 arrest) exhibited high NTCYC1 transcript levels.

Conclusions:

  • NTCYC1 expression is cell cycle-dependent, specifically during G2/M phases.
  • NTCYC1 plays a role in regulating the G2/M transition in plant cells.
  • NTCYC1 expression levels correlate with naturally occurring G2-arrested cell populations in planta.

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