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Developmental and tissue-specific sulfonylurea receptor gene expression

C Hernández-Sánchez1, T L Wood, D LeRoith

  • 1Section on Molecular and Cellular Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-1770, USA.

Endocrinology
|February 1, 1997
PubMed
Summary

The sulfonylurea receptor (SUR) gene is expressed early in mouse development across multiple tissues, including the pancreas and brain. This widespread expression suggests diverse roles for SUR beyond pancreatic islets.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The sulfonylurea receptor (SUR) is a key component of ATP-sensitive potassium channels.
  • Understanding the developmental expression pattern of SUR is crucial for elucidating its physiological roles.

Purpose of the Study:

  • To investigate the developmental regulation of mouse SUR gene expression.
  • To identify tissues and developmental stages where SUR is expressed.

Main Methods:

  • In situ hybridization
  • Solution hybridization/ribonuclease protection assays
  • Utilized a 229-bp mouse complementary DNA probe for the 3'-end of the SUR gene.

Main Results:

  • SUR gene expression was detected as early as embryonic day 12 in the pancreas, heart, and central nervous system.

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  • Significant SUR messenger RNA (mRNA) levels were maintained throughout development in these tissues.
  • Widespread SUR mRNA distribution was observed in adult mouse tissues, notably high in brain regions like the cerebellum, hippocampus, and thalamus, with an exception in the liver. In adult pancreas, expression was exclusive to endocrine tissue.

    • Conclusions:

    • The SUR gene exhibits broad developmental expression in mice.
    • SUR mRNA is widely distributed in adult tissues, indicating potential functions beyond pancreatic islets.
    • The specific expression patterns suggest critical roles for SUR in various organ systems during development and in adulthood.