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The Schizosaccharomyces pombe map1 gene encodes an SRF/MCM1-related protein required for P-cell specific gene

O Nielsen1, T Friis, S Kjaerulff

  • 1Department of Genetics, University of Copenhagen, Denmark.

Molecular & General Genetics : MGG
|December 13, 1996
PubMed

Insights

In Schizosaccharomyces pombe, the map1 gene and mat1-Pc gene are essential for P-specific gene expression during mating and meiosis. These genes encode transcription factors that may form a heterodimer to activate P-specific gene map3.

Area of Science:

  • Cellular and Molecular Biology
  • Yeast Genetics
  • Transcriptional Regulation

Background:

  • Schizosaccharomyces pombe cells engage in mating and meiosis under nitrogen starvation.
  • P mating type determination involves the mat1-Pc gene and the map1 gene.
  • These factors regulate the expression of P-specific genes like map3.

Purpose of the Study:

  • To investigate the roles of mat1-Pc and map1 in P-specific gene expression.
  • To clone and characterize the map1 gene.
  • To elucidate the mechanism of map3 gene activation.

Main Methods:

  • Gene cloning and characterization of map1.
  • Analysis of gene expression patterns.
  • Protein interaction studies.

Main Results:

  • The map1 gene was cloned and identified as encoding a MADS-box transcription factor.
  • Map1 is related to mammalian SRF and yeast MCM1.
  • Mat1-Pc protein possesses a motif for interaction with MADS-box factors.

Conclusions:

  • Map1 and Mat1-Pc likely form a heterodimer.
  • This heterodimer is crucial for activating the P-specific map3 gene.
  • Provides insights into mating-type specific gene regulation in S. pombe.

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