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Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells
T W Gardner1, T Lesher, S Khin
1Ulerich Ophthalmology Research Laboratory, Department of Ophthalmology, Penn State University College of Medicine, Hershey, PA 17033, USA.
The Biochemical Journal
|December 15, 1996
Abstract:
We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10(-9) -10(-4) M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.