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Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells
C Decker1, M J Miro Obradors, D J Sillence
1Department of Physiology, University College London, U.K.
The Biochemical Journal
|December 15, 1996
Abstract:
The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated with phorbol 12-myristate 13-acetate and n-butanol. Results derived by isolation of plasma membrane vesicles from intact cells or by subcellular fractionation on a sucrose density gradient suggest the PLD is specific for phosphatidylcholine and its primary site of action is not the plasma membrane but the endoplasmic reticulum.