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Related Experiment Videos

Evidence for a negative Pasteur effect in articular cartilage

R B Lee1, J P Urban

  • 1University Laboratory of Physiology, University of Oxford, U.K.

The Biochemical Journal
|January 1, 1997
PubMed
Summary

Articular cartilage exhibits a negative Pasteur effect, meaning low oxygen severely reduces energy production from glucose. This makes chondrocytes vulnerable to energy deficits in anoxic conditions.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Orthopedics

Background:

  • Articular cartilage is avascular and relies on anaerobic glycolysis for energy.
  • Chondrocytes, the cartilage cells, must maintain ATP levels for essential functions like proteoglycan synthesis.

Purpose of the Study:

  • To investigate the metabolic response of bovine articular cartilage to varying oxygen levels.
  • To determine the role of glycolysis and ATP production in cartilage energy metabolism.
  • To assess the impact of anoxia on proteoglycan synthesis.

Main Methods:

  • Measurement of glucose uptake and lactate production in bovine articular cartilage under different oxygen concentrations.
  • Use of glycolytic inhibitors (2-deoxy-D-glucose, iodoacetamide, fluoride) and mitochondrial inhibitors (azide, cyanide).
  • Assessment of [35S]sulphate incorporation into proteoglycan.

Main Results:

  • Anoxia significantly inhibited glucose uptake and lactate production, indicating a negative Pasteur effect.
  • Lactate production decreased proportionally with glucose uptake (2:1 mole ratio).
  • Glycolytic inhibitors reduced aerobic lactate production and ATP levels.
  • Anoxia and ATP-depleting treatments curtailed proteoglycan synthesis.

Conclusions:

  • Bovine articular cartilage demonstrates a negative Pasteur effect, relying heavily on glycolysis for ATP.
  • Chondrocytes are susceptible to energy shortages under anoxic conditions.
  • Adequate ATP levels are crucial for proteoglycan synthesis in articular cartilage.

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