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Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina
E O Omolo1, M D James, E O Osir
1The International Center of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya.
Current Microbiology
|February 1, 1997
Abstract:
A local isolate of Bacillus thuringiensis,designated L1-2, that is toxic to Chilo partellus was found to be toxic to the adult tsetse fly, Glossina morsitans morsitans. The delta-endotoxin crystals derived from the isolate gave a major protein band with a molecular weight of Mr 130,000-140,000 on denaturing polyacrylamide gel electrophoresis. The sequence of the cloned gene was found to be similar to that of the B. thuringiensis subsp. kurstaki HD-73 cryIA(c) gene, having one amino acid difference at position 148 and four additional DNA differences.