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A new approach to labeling cells with technetium-99m, Part II. Evaluation of radiolabeled human-lymphocyte immune
L I Delmon-Moingeon1, A Mahmood, A Davison
1Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA USA.
Abstract:
Technetium-99m-modified polylysine (MPL) is a mild and efficient method for cell labeling that is easily applicable to human lymphocytes. 99mTc-MPL uptake is maximal in 40 min at room temperature. Cell labeling efficiency (from 60 to 80%) increases with rising concentrations of cells and labeling agent. In vitro stability of 99mTc-MPL on lymphocytes incubated in serum at 37 degrees C is high. Microautoradiography indicates that 99mTc-MPL is equally distributed within the cells. The radiolabeling procedure does not alter expression of surface receptors involved in T lymphocyte effector functions, adhesion function, cytolytic activity or proliferative response to IL-2.