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Reversed-phase high-performance liquid chromatographic study of thimerosal stability in Cuban recombinant hepatitis B
D Tleugabulova1, I González Perez
1National Center for Bioproducts, Havana, Cuba.
Journal of Chromatography. A
|April 5, 1996
Abstract:
Non-degraded thimerosal was determined in the presence of its decomposition products by directly assaying recombinant hepatitis B vaccine using a reversed-phase liquid chromatographic method. Methanol-water-orthophosphoric acid (65:35:0.9, v/v/v) was used as the eluent. Salicylic acid was employed as an internal standard. The calibration graph was linear (r = 0.99995) up to 2.5 micrograms of thimerosal. Interference from aluminium hydroxide was eliminated by centrifugation. Good stability of thimerosal in the hepatitis B vaccine was demonstrated. The results obtained were in agreement with the recently proposed mechanism of degradation.