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Automatic high-performance liquid chromatographic method for the determination of diarrhetic shellfish poison
C Hummert1, J L Shen, B Luckas
1Institute of Nutrition and Environment, Friedrich-Schiller-University of Jena, Germany.
Journal of Chromatography. A
|April 5, 1996
Abstract:
A rapid HPLC method with fluorescence detection for the determination of okadaic acid (OA) and dinophysistoxin-1 (DTX-1) in mussels and mussel products is presented. For fluorescence labelling of OA and DTX-1, 9-anthryldiazomethane (ADAM) is used. HPLC with a column-switching system is proposed to avoid time-consuming clean-up procedures after derivatization of sample extracts with ADAM. The column-switching system as well as the chromatographic conditions and detection are described.