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Updated: Aug 2, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[A comparative study of the effectiveness of commercial microtest systems for identification of microorganisms of
Abstract:
Studies of 806 strains of cultures isolated from pathological material demonstrated the possibility of using microtest systems MMTE1 and 2 (ALLERGEN Research and Production Plant in the town of Stavropol) and ENTEROtest 1 and 2 and ENTEROtest 16 for identification of enterobacteria and NEFERMtest, STAPHYtest, STREPTOtest, and ANAEROtest for the identification of respective groups of microorganisms at practical microbiological laboratories (Lachema, Czechia). The microtest kits are easy to use and fit for mass screenings; they permit simultaneous testing in 9 to 23 biochemical tests. Use of these test kits allows species identification of 63.3 to 80.7% cultures making use of biochemical activity tables and indexes recommended in instructions for the use of microtest kits and 85.6 to 96.1% cultures making use of IDENT computer software. Introduction of microtest kits in the practical activity of microbiological laboratories will appreciably improve the quality of microbiological investigations and allow the use of automated microbiological systems.
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