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Related Experiment Videos

Micropatterning fluid lipid bilayers on solid supports

J T Groves1, N Ulman, S G Boxer

  • 1Department of Chemistry, Stanford University, Stanford, CA 94305, USA. University, Stanford, CA 94305, USA.

Science (New York, N.Y.)
|January 31, 1997
PubMed
Summary
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Researchers created isolated fluid membrane corrals using lithographic patterning. These corrals allow controlled manipulation of lipid concentrations and provide a parallel method for studying membrane properties like diffusion.

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Supported lipid bilayers are crucial models for cell membranes.
  • Controlling membrane composition and dynamics at the microscale is essential for understanding cellular processes.
  • Existing methods for creating compositional gradients are often limited in parallelization and resolution.

Purpose of the Study:

  • To develop a method for creating arrays of isolated, fluid membrane corrals.
  • To demonstrate the ability to control lipid composition within individual corrals.
  • To establish a parallel platform for investigating membrane molecular properties.

Main Methods:

  • Utilized lithographically patterned grids (photoresist, Al2O3, Au) on silicon substrates to define membrane corrals.

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  • Employed fluorescently labeled lipids to visualize and track diffusion within corrals.
  • Applied selective photobleaching to alter probe concentrations and create compositional gradients.
  • Used electric fields parallel to the surface to induce steady-state concentration gradients of charged membrane components.

    • Main Results:

    • Successfully partitioned supported lipid bilayers into micrometer-scale isolated fluid membrane corrals.
    • Observed free diffusion of lipids within corrals, confined by micropatterned barriers.
    • Created arrays of membrane patches with varying compositions via photobleaching.
    • Induced steady-state concentration gradients of charged membrane components using electric fields.

    Conclusions:

    • Lithographic patterning provides precise control over membrane corral formation and isolation.
    • Photobleaching and electric fields offer effective methods for manipulating membrane composition and creating gradients.
    • This platform enables parallel acquisition of data on molecular properties, such as diffusion coefficients, within individual membrane corrals.