Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse disabled (mDab1): a Src binding protein implicated in neuronal development

B W Howell1, F B Gertler, J A Cooper

  • 1Fred Hutchinson Cancer Research Center Seattle, WA 98104, USA.

The EMBO Journal
|January 2, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE.

Oncogene·2016
Same author

The Use and Abuse of Plaster of Paris.

Postgraduate medical journal·2011
Same author

Deformities of the Foot in Children: The Treatment of those Types due to Affections of the Nervous System.

Postgraduate medical journal·2011
Same author

TYPHUS IN SERBIA.

British medical journal·2010
Same author

THE TREATMENT OF CONGENITAL CLUB-FOOT.

British medical journal·2010
Same author

THE TREATMENT OF TORTICOLLIS.

British medical journal·2010

Researchers identified a mouse Disabled (Dab) protein homolog, mDab1, crucial for neural development. This adaptor molecule binds to phosphotyrosine proteins and interacts with Src kinases during nerve formation.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • The Drosophila Disabled (Dab) protein is implicated in neural development.
  • Adaptor proteins play critical roles in signal transduction pathways.
  • Understanding conserved molecular mechanisms across species is vital for neuroscience.

Purpose of the Study:

  • To identify and characterize a mouse homolog of the Drosophila Disabled (Dab) protein.
  • To elucidate the function of the mouse Disabled protein (mDab1) in neural development.
  • To investigate the molecular interactions and signaling pathways involving mDab1.

Main Methods:

  • Identification and expression analysis of the mDab1 gene in mouse cell lines and embryonic tissues.
  • Immunoprecipitation and Western blotting to detect mDab1 phosphorylation and protein interactions.

Related Experiment Videos

  • Analysis of mDab1's phosphotyrosine binding (PTB) domain interactions with cellular proteins.

    • Main Results:

    • A mouse homolog of Drosophila Disabled (Dab), termed mDab1, was identified.
    • mDab1 is expressed in neuronal and hematopoietic cells and localizes to developing nerve structures.
    • mDab1 undergoes tyrosine phosphorylation during embryonic nervous system expansion and associates with Src family kinases (Src, Fyn, Abl).
    • The mDab1 PTB domain binds to specific phosphotyrosine-containing proteins in embryonic mouse brain extracts.

    Conclusions:

    • mDab1 functions as an adaptor molecule in neural development, analogous to Drosophila Dab.
    • mDab1's interactions with Src kinases and phosphotyrosine proteins suggest its role in neural network formation.
    • These findings highlight conserved signaling pathways in neural development across species.