Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformational dynamics in cytochrome P450-substrate interactions

H Li1, T L Poulos

  • 1Department of Molecular Biology and Biochemistry, University of California at Irvine 92697, USA.

Biochimie
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenobarbital induction mediated by a distal CYP2B2 sequence in rat liver transiently transfected in situ.

The Journal of biological chemistry·1996
Same author

Induction of phosphoglycerate kinase 1 gene expression by hypoxia. Roles of Arnt and HIF1alpha.

The Journal of biological chemistry·1996
Same author

Identification of novel regions of deletion in familial Wilms' tumor by comparative genomic hybridization.

Cancer research·1996
Same author

Alternatively spliced cyclin C mRNA is widely expressed, cell cycle regulated, and encodes a truncated cyclin box.

Oncogene·1996
Same author

Emergence of preferred structures in a simple model of protein folding.

Science (New York, N.Y.)·1996
Same author

Developmental regulation of collagen differential expression in the rabbit bladder.

The Journal of urology·1996
Same journal

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Biochimie·2026
Same journal

Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

Biochimie·2026
Same journal

Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

Biochimie·2026
Same journal

Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

Biochimie·2026
Same journal

Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

Biochimie·2026
Same journal

Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

Biochimie·2026
See all related articles

Researchers analyzed the P450BM-3 heme domain, revealing significant conformational changes in the substrate access channel upon binding palmitoleic acid. This provides key insights into cytochrome P450 substrate recognition mechanisms.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Enzymology

Background:

  • Four cytochrome P450 crystal structures are known.
  • P450cam and P450eryF are substrate-bound, while P450terp and P450BM-3 heme domain are substrate-free.

Purpose of the Study:

  • To analyze the P450BM-3 heme domain in complex with palmitoleic acid.
  • To compare substrate-free and substrate-bound structures to understand conformational changes.

Main Methods:

  • Preliminary crystallographic analysis of the P450BM-3 heme domain.
  • Comparison of structural data between substrate-free and palmitoleic acid-bound states.

Main Results:

  • A large conformational change in the substrate access channel was observed upon palmitoleic acid binding.

Related Experiment Videos

  • Structural comparison highlights critical regions for substrate binding dynamics.

    • Conclusions:

    • Substrate binding induces significant conformational alterations in P450BM-3.
    • Understanding these dynamics is crucial for deciphering P450 substrate recognition.