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Updated: Aug 5, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Effect of sperm-agglutinating antibodies on sperm capacitation and acrosome reaction
Objective:
To study the effect of polyclonal/monospecific antisera on sperm agglutination versus capacitation as well as acrosome reaction.
Materials And Methods:
Swim-up spermatozoa from cauda epididymides of fertile male hamsters were incubated under liquid paraffin with polyclonal/monospecific antisera obtained from immunized BALB/C mice, as well as with normal serum from control BALB/C mice, at various dilutions.
Results:
The anti-sperm antibodies caused a significant (P < .05) sperm agglutination of various types of dilutions below 1:1000. Both capacitation and true acrosome reaction were inhibited significantly in the spermatozoa incubated with polyclonal/monospecific antisera. Capacitation in the spermatozoa with normal serum started earlier, i.e., at 2 hours of incubation compared to 3 hours of incubation in controls.
Conclusion:
The data differentiate the sperm agglutinating activity from anticapacitation and antiacrosome reaction activity of antisperm antisera at 1:1000 dilution.
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