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Related Experiment Videos

Embryoglycans regulate FGF-2-mediated mesoderm induction in the rabbit embryo

P Dvorák1, J E Flechon, E M Thompson

  • 1Developmental Biology Unit, Institute of Animal Physiology and Genetics, Czech Republic.

Journal of Cell Science
|January 1, 1997
PubMed
Summary

Embryonic cell surface epitopes, specifically TEC 1, regulate fibroblast growth factor-2 (FGF-2) binding during mesoderm formation. TEC 1 redistribution indicates early mesoderm development in rabbit embryos.

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Area of Science:

  • Developmental biology
  • Cell signaling
  • Glycobiology

Background:

  • Fibroblast growth factors (FGFs) are key regulators of mesoderm induction.
  • Proteoglycans and embryoglycans are involved in growth factor binding.
  • The TEC 1 epitope is a developmentally regulated marker on embryonic cell surfaces.

Purpose of the Study:

  • To investigate the role of embryoglycans carrying the TEC 1 epitope in FGF-2 binding.
  • To determine if TEC 1 is involved in FGF-2 mediated mesoderm differentiation.
  • To explore the temporal and spatial regulation of TEC 1 during embryonic development.

Main Methods:

  • Culture of rabbit inner cell masses (ICMs) with FGF-2.
  • Immunohistochemical staining for the TEC 1 epitope.

Related Experiment Videos

  • Inhibition studies using antibodies against TEC 1.
  • Analysis of TEC 1 staining patterns in vivo and in vitro.

    • Main Results:

    • FGF-2 induced differentiation of ICMs into mesoderm-like cells.
    • TEC 1 epitope redistribution from cell membranes to an apical pattern during differentiation.
    • Antibodies against TEC 1 blocked FGF-2 binding and differentiation.
    • TEC 1 redistribution observed in vivo parallels in vitro findings.

    Conclusions:

    • Embryoglycans carrying the TEC 1 epitope regulate FGF-2 receptor binding.
    • TEC 1 binding activity is transient, restricted to ectodermal cells, and diminishes during differentiation.
    • Apical redistribution of TEC 1 serves as an early indicator of mesoderm formation.