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Purification of measles virus with preservation of infectivity and antigenicity
Abstract:
Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.
Insights
This study presents an effective purification method for measles virus (Edmonston strain), yielding highly pure virus preparations suitable for immunological studies and vaccine development. The method ensures viral infectivity and antigenicity for antibody production.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Measles virus is a significant human pathogen requiring effective purification methods for research.
- High-purity viral antigens are crucial for developing sensitive diagnostic tools and effective vaccines.
Purpose of the Study:
- To develop and validate a robust purification protocol for the Edmonston strain of measles virus.
- To assess the purity, infectivity, and immunogenicity of the purified measles virus preparations.
Main Methods:
- Ultrafiltration and sucrose density gradient centrifugation were employed for virus purification.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze viral protein composition.
- Immunochemical assays with specific antisera confirmed the purity of the measles virus preparations.
Main Results:
- The purification method successfully yielded milligram quantities of measles virus.
- Purified virus preparations retained infectivity and elicited high-titered antibodies against measles antigens.
- SDS-PAGE and immunochemical analyses confirmed the high purity of the isolated virus.
Conclusions:
- The described purification strategy is effective for obtaining relatively pure measles virus suitable for immunological applications.
- This method facilitates the production of measles virus antigens for serological testing and vaccine research.
- The findings support the utility of this protocol in virology and vaccine development efforts.