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Endogenous histamine in rabbit thoracic aorta

A Foldes, R J Head, I S de la Lande

    The Australian Journal of Experimental Biology and Medical Science
    |February 1, 1977
    PubMed
    Summary
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    Researchers developed a sensitive assay to measure histamine in rabbit aorta. They found histamine uptake in the aorta is an active, temperature-dependent process influenced by specific inhibitors.

    Area of Science:

    • Pharmacology
    • Biochemistry
    • Physiology

    Background:

    • Histamine plays a crucial role in various physiological processes.
    • Understanding histamine transport mechanisms in vascular tissues is essential.

    Purpose of the Study:

    • To develop a sensitive automated assay for histamine determination.
    • To investigate the endogenous histamine content and uptake mechanisms in rabbit thoracic aorta.

    Main Methods:

    • Development of a sensitive automated assay for histamine quantification.
    • Determination of histamine in intact and separated layers of rabbit thoracic aorta.
    • Investigation of radiolabeled histamine uptake in isolated aorta under various conditions (temperature, oxygen, inhibitors).

    Main Results:

    Related Experiment Videos

    • The histamine content of the intact aorta showed partial sensitivity to compound 48/80.
    • Isolated rabbit aorta actively accumulated labeled histamine against a concentration gradient.
    • Uptake was temperature-dependent (abolished at 1°C) but independent of oxygen.
    • The uptake process was inhibited by extraneuronal catecholamine uptake inhibitors and histamine/catecholamine metabolism inhibitors, but not by a histamine N-methyl-transferase inhibitor or specific metabolites.

    Conclusions:

    • A sensitive automated assay for histamine was successfully developed.
    • Rabbit thoracic aorta exhibits an active, temperature-sensitive mechanism for histamine uptake.
    • This uptake process shares characteristics with extraneuronal catecholamine transport and is influenced by metabolic pathways.