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Related Experiment Videos

Alpha N-catenin expression in the normal and regenerating chick sciatic nerve

Y Shibuya1, H Yasuda, M Tomatsuri

  • 1Department of Oral and Maxillofacial Surgery, Kobe University School of Medicine, Japan.

Journal of Neurocytology
|November 1, 1996
PubMed
Summary

Alpha N-catenin, a protein linked to cell adhesion, is found throughout the cytoplasm of regenerating axons in chick sciatic nerve, not just on the cell surface. This suggests it

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Cadherin proteins mediate calcium-dependent cell adhesion.
  • Catenin proteins link cadherins to the cytoskeleton, crucial for cell adhesion.
  • N-cadherin and alpha N-catenin are specifically associated with N-cadherin.

Purpose of the Study:

  • To investigate the immunolocalization of alpha N-catenin in normal and regenerating chick sciatic nerve.
  • To determine the subcellular distribution of alpha N-catenin in relation to N-cadherin and the cytoskeleton.

Main Methods:

  • Immunohistochemistry was used to detect alpha N-catenin.
  • Chick sciatic nerves (normal and regenerating) were examined.

Main Results:

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  • Alpha N-catenin was found throughout the axoplasm of unmyelinated axons in normal nerve.
  • Myelinated axons in normal nerve were immunonegative for alpha N-catenin.
  • Regenerating axons, including growth cones and axon shafts, showed variable alpha N-catenin immunoreactivity.
  • Schwann cells were consistently devoid of alpha N-catenin immunoreactivity.

Conclusions:

  • Alpha N-catenin is distributed throughout the cytoplasm of axons, not solely localized to the plasmalemma.
  • The cytoplasmic localization suggests that most alpha N-catenin molecules are not bound to N-cadherin in these axonal populations.
  • This finding provides insights into the dynamic interactions of catenins and cadherins during nerve regeneration.