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Preparation and characterization of immunostimulating complexes (ISCOMs) of Japanese encephalitis virus
1National Institute of Virology, Maharashtra, India.
ISCOMs (immunostimulating complexes) were prepared from envelope glycoprotein (Egp) of Japanese encephalitis (JE) virus. ISCOMs showed a single band of the viral Egp in SDS-PAGE, which reacted with polyclonal and monoclonal antibody (MAb) raised against Egp. Comparison between the epitopes exposed on JE virion and JE ISCOMs, by antigen capture ELISA, utilizing a panel of domain-specific MAbs, revealed identical epitopes exposed on the Egp incorporated in ISCOMs and the whole virion. Electron micrographs of ISCOMs showed spherical cage-like structures of 35 nm. ISCOMs with Egp were good immunogenes, which stimulated high titres of neutralizing antibodies, both in mice and rabbits.
ISCOMs (immunostimulating complexes) were prepared from envelope glycoprotein (Egp) of Japanese encephalitis (JE) virus. ISCOMs showed a single band of the viral Egp in SDS-PAGE, which reacted with polyclonal and monoclonal antibody (MAb) raised against Egp. Comparison between the epitopes exposed on JE virion and JE ISCOMs, by antigen capture ELISA, utilizing a panel of domain-specific MAbs, revealed identical epitopes exposed on the Egp incorporated in ISCOMs and the whole virion. Electron micrographs of ISCOMs showed spherical cage-like structures of 35 nm. ISCOMs with Egp were good immunogenes, which stimulated high titres of neutralizing antibodies, both in mice and rabbits.