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Immunisation regimes used to elevate serum IgE in sheep
H Yilmaz1, J M Roe, K L Morgan
1University of Istanbul, Veterinary Faculty, Department of Microbiology, Avcilar, Turkey.
Veterinary Immunology and Immunopathology
|December 1, 1996
Summary
Researchers explored immunisation strategies to boost ovine immunoglobulin E (IgE) levels. The most effective method involved Ascaris infection combined with parenteral Ascaris antigen administration, achieving high IgE titres.
Area of Science:
- Veterinary Immunology
- Parasitology
Background:
- Ovine immunoglobulin E (IgE) plays a role in allergic responses.
- Understanding methods to elevate IgE is crucial for immunological studies.
Purpose of the Study:
- To evaluate different immunisation regimes for increasing ovine IgE concentration.
- To identify the most effective method for inducing a high IgE titre in sheep.
Main Methods:
- Six distinct immunisation protocols were tested.
- Protocols included Ascaris suum infection and parenteral administration of Ascaris antigens and ovalbumin with adjuvants.
- Passive cutaneous anaphylaxis (PCA) tests were used to measure IgE response.
Main Results:
- The combination of Ascaris infection and parenteral Ascaris extract in Bordetella pertussis yielded the highest IgE titres.
- This regime consistently produced transient high serum IgE titres (1:1280-1:2560) 9-12 days post-immunisation.
- Ovalbumin induced a transient IgE response with a maximum PCA titre of 1:10.
Conclusions:
- A combined immunisation strategy of Ascaris infection and parenteral antigen administration is highly effective for elevating ovine IgE.
- This approach provides a reliable method for inducing significant, albeit transient, IgE responses in sheep.