Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rotavirus subunit vaccines

M E Conner1, S E Crawford, C Barone

  • 1Veterans Administration Medical Center, Baylor College of Medicine, Houston, Texas, USA.

Archives of Virology. Supplementum
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial intelligence entering the pathology arena in oncology: current applications and future perspectives.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

Comparison of "IN-REC-SUR-E" and LISA in preterm neonates with respiratory distress syndrome: a randomized controlled trial (IN-REC-LISA trial).

Trials·2024
Same author

Socially constructed beliefs and the uptake of the Child Dental Benefits Schedule.

Australian dental journal·2024
Same author

Effect of the substrate on the electrical transport and fluctuation processes in NbRe and NbReN ultrathin films for superconducting electronics applications.

Scientific reports·2022
Same author

Transport mechanisms in Co-doped ZnO (ZCO) and H-irradiated ZCO polycrystalline thin films.

Physical chemistry chemical physics : PCCP·2021
Same author

Conductivity response of amorphous oxide interfaces to pulsed light illumination.

Nanotechnology·2019
Same journal

Filoviruses. A compendium of 40 years of epidemiological, clinical, and laboratory studies.

Archives of virology. Supplementum·2008
Same journal

Structural biology of old world and new world alphaviruses.

Archives of virology. Supplementum·2005
Same journal

Ehrlichia under our noses and no one notices.

Archives of virology. Supplementum·2005
Same journal

The spread of the H5N1 bird flu epidemic in Asia in 2004.

Archives of virology. Supplementum·2005
Same journal

Control of arbovirus diseases: is the vector the weak link?

Archives of virology. Supplementum·2005
Same journal

Host range, amplification and arboviral disease emergence.

Archives of virology. Supplementum·2005
See all related articles

Rotavirus virus-like particles (VLPs) show promise as subunit vaccines. Parenteral immunization with VLPs induced protective immunity in rabbits, mice, and cattle, suggesting potential for broad protection with limited VLP types.

Area of Science:

  • Virology
  • Immunology
  • Vaccine Development

Background:

  • Rotaviruses are a leading cause of severe diarrheal disease in humans and animals.
  • Current rotavirus vaccines have limitations, necessitating the development of alternative strategies.
  • Virus-like particles (VLPs) are non-infectious, self-assembling protein structures that mimic native viruses and can serve as potent immunogens.

Purpose of the Study:

  • To evaluate the immunogenicity and protective efficacy of rotavirus virus-like particles (VLPs) as subunit vaccines.
  • To assess the potential for inducing broad heterotypic immunity using limited VP7 protein types or chimeric VLPs.
  • To determine the effectiveness of VLPs in different animal models, including rabbits, mice, and dairy cattle.

Main Methods:

  • Insect cells were co-infected with baculovirus recombinants to produce VLPs expressing rotavirus structural proteins (VP2, VP4, VP6, VP7).

Related Experiment Videos

  • VLPs were administered parenterally to rabbits, mice, and dairy cows to assess immune responses and protection against rotavirus challenge.
  • Neutralizing antibody responses and protection against homologous and heterotypic rotavirus challenge were evaluated.

    • Main Results:

    • Parenteral immunization with rotavirus VLPs induced complete or partial protection against rotavirus challenge in rabbits.
    • VLPs containing G1 VP7 induced broadly reactive neutralizing antibodies in mice, suggesting potential for heterotypic immunity.
    • VLPs were effective in boosting lactogenic immunity in dairy cows, and G3 VLPs showed potential as a bovine subunit vaccine.

    Conclusions:

    • Rotavirus VLPs are effective immunogens when administered parenterally in various animal models.
    • A limited number of VLP types may be sufficient to elicit broadly protective immune responses.
    • Rotavirus VLPs represent a promising platform for developing effective human and animal vaccines against rotavirus infections.