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Related Experiment Videos

Structure of Norwalk virus

B V Prasad1, M E Hardy, X Jiang

  • 1Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas, USA.

Archives of Virology. Supplementum
|January 1, 1996
PubMed
Summary

Researchers visualized the Norwalk virus capsid structure using electron cryomicroscopy. This breakthrough reveals the virus-like particle

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Area of Science:

  • Virology
  • Structural Biology
  • Biochemistry

Background:

  • Norwalk virus is a leading cause of human epidemic viral gastroenteritis.
  • Culturing Norwalk virus in laboratory settings has proven challenging.
  • Recombinant baculovirus expression in insect cells enables spontaneous assembly of Norwalk virus-like particles.

Purpose of the Study:

  • To determine the 3-dimensional structure of baculovirus-expressed Norwalk virus.
  • To elucidate the structural organization of the Norwalk virus capsid protein.

Main Methods:

  • Electron cryomicroscopy (cryo-EM) and computer image reconstruction were employed.
  • Structural analysis was performed on virus-like particles expressed in insect cells.
  • High-resolution X-ray crystallography is being pursued using crystallized virus particles.

Main Results:

  • The 3-dimensional structure of Norwalk virus-like particles was determined to approximately 22 A resolution.
  • These particles exhibit T = 3 icosahedral symmetry with a diameter of 380 A.
  • The structure comprises 90 dimers of the 58,000 molecular weight capsid protein, forming arch-like capsomeres with modular domains.

Conclusions:

  • The study provides a high-resolution structural model of the Norwalk virus capsid.
  • Understanding the capsid structure is crucial for developing antiviral strategies.
  • The successful crystallization of virus-like particles opens avenues for atomic-level structural determination.

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