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Related Experiment Videos

SF-assemblin in Chlamydomonas: sequence conservation and localization during the cell cycle

K F Lechtreck1, C D Silflow

  • 1Botanisches Institut der Universitat zu Koln, Germany.

Cell Motility and the Cytoskeleton
|January 1, 1997
PubMed
Summary

SF-assemblin, a key protein in striated microtubule-associated fibers (SMAFs), undergoes significant sequence changes in green algae. Its dynamic reorganization during mitosis suggests a role in rebuilding the microtubular root system post-cell division.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeleton Dynamics

Background:

  • SF-assemblin is the filament-forming protein of striated microtubule-associated fibers (SMAFs) in green flagellates.
  • SMAFs originate from basal bodies and are crucial for flagellar structure.

Purpose of the Study:

  • To sequence SF-assemblin cDNAs from Chlamydomonas reinhardtii and Chlamydomonas eugametos.
  • To investigate the dynamic behavior of SF-assemblin during the cell cycle, particularly mitosis.

Main Methods:

  • cDNA sequencing and amino acid sequence comparison.
  • Double immunofluorescence microscopy using anti-SF-assemblin and anti-alpha-tubulin antibodies.

Main Results:

  • SF-assemblin sequences show 54-71% identity among green algal species, indicating sequence drift.

Related Experiment Videos

  • In interphase cells, SF-assemblin localizes to all four microtubular flagellar roots.
  • During mitosis, SF-assemblin undergoes reorganization, dividing, reducing to dots at spindle poles, and reforming fibers in telophase.

    • Conclusions:

    • SF-assemblin exhibits significant sequence variation across related species.
    • The dynamic localization of SF-assemblin during mitosis suggests a critical role in the reformation of the microtubular root system after cell division.